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a robust multi-gene cloning method

multi-gene cloning

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2 replies to this topic

#1 clwop

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Posted 01 February 2012 - 11:47 PM

Here is a robust multi-gene cloning method developped in our lab. it is easy to use and require nothing special. The efficiency is much higher than that of restriction-ligation based methods. http://dx.plos.org/1...al.pone.0030267
please return your comments.

#2 phage434

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Posted 02 February 2012 - 06:11 AM

This is very nice work. I would be interested to know your approach to repetitive sequence assembly, such as a construct with two closely related variants of GFP, for example.

#3 scolix

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Posted 02 February 2012 - 08:50 AM

Really interesting. I would like to see how they behave with viral vectors construction where there are inverted repeats like in AAV or lentivirus plasmids.




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