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Double strand cDNA sysnthesis


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#1 Paul

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Posted 02 May 2003 - 09:17 AM

I wish to synthesis double-strand cDNA from total RNA using RANDOM primers. What is the best kit to use. Also how can i monitor the synthesis of cDNA without using radiolabelled nucleotides?

#2 zhenghj

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Posted 09 May 2003 - 02:18 AM

Try Stratagene.It's difficult to check ur synthesis result in the process of synthesis.
Based on my experience,if ur RNA is good enough,go on ur synthesis until the step of ligation with vector,then u can check the amount of cDNA .




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