Posted 01 May 2003 - 10:45 PM
i tried to do northern, my problem is when i exposed the filter(nytran) to the film i got dark picture. i assume that the prob binded unspecificlly but i used salmon sperm, i baked the filter in uv 3 min. each side and i washed the filter 5 times with different concentration of sds and ssc.
any more suggestion why the prob can bind unspecificlly to the filter?
thank is advence
Posted 05 May 2003 - 03:26 AM
Yuo're not alone!
I think I have the same problem. Even after stripping I cannot get rid of the background. I prehybridized and hybridized according to the protocol and now I still have background.....
Posted 27 June 2003 - 10:30 AM
Do you mean your whole film was dark? or there was dark stains somewhere in the film?
Posted 28 June 2003 - 06:19 AM
if u have more suggestions i will glad to hear about them