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Analysis of high throughput screen for cell surface markers

cell surface flow cytometry

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#1 kuby007



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Posted 31 January 2012 - 12:12 PM


I'm totally new to the world of flow cytometry data analysis and am looking for some help. I have 4 cell lines that were analyzed by high throughput screen flow cytometry for over 300 CD and other surface molecules. I received an excel file with MFI and %gate+ for each molecule for each cell line. I want to compared the level of expression between the cell lines. My questions are:

1) How do I normalized the data (I do not have a control or anything)
2) Do I use MFI or %gate+ for the anaylsis. For either, what is a good cut-off value (i.e. what is background level?)
3) How do I normalize the data?

Any insight would be greatly appreciated!

#2 scolix


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Posted 01 February 2012 - 12:34 AM

Do all of your cells express the proteins of interest. There might be some negative cell lines and maybe you could use them for comparison.

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