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Number of snap per hour in time laps system

Started by ThibautEBV, Jan 30 2012 05:26 AM

Time laps Epifluorescence snap

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#1 ThibautEBV

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Posted 30 January 2012 - 05:26 AM

Hello,

I'm going to do some time laps experiments using HONE cell line and I'm wondering how many snaps I can take per hour. More precisely I'm wondering how many fluorescence exposition i can do per hour (24h total) to avoid or have small phototoxicity. If you have any average it could be a great help.

Thanks in advance.

Thibaut

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#2 bob1

Thelymitra pulchella

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Excellent

Posted 30 January 2012 - 02:05 PM

It depends on what you are wanting to see - migration of cells? transport of a protein? cell divisons?... The biggest problem with fluorescence is the heat generated by the bulb running, and potential photo-bleaching of your fluorophore with the light running.