Problem with soft agar assay
Posted 29 January 2012 - 02:27 PM
However, now I came across a bizzare problem. I can see many cells adhered to the plate within a few days of cell plating and growing in monolayer instead of forming typical three-dimensional colonies. I know I had no problem in pouring the agar. These are breast cancer cells. Bottom layer is 2.5 ml of 0.5% Agar Noble (in DMEM) per well and top agar is 1 ml of 0.33% Agar Noble per well (with 25,000 to 100,000 cells in MEGM).
Somebody in this forum had posted similar problem in August 2010, which got no attention or reply from anybody. Please help me out here if you guys have any thoughts.
Posted 31 January 2012 - 11:41 AM
Posted 31 January 2012 - 12:49 PM