i did a double digest with Ecor1 & BamH1 with a 7Kb plasmid sample prepared through miniprep but not purified.
in 10ul reaction volume i used 2 ul DNA ,1ul10xBSA ,1ulNEbuffer4 and 0.2ul of each enz.
got no expected fragment . these were plasmids taken from transformants of the ligation mix of backbone + fragment.
Edited by angadbiochem, 28 January 2012 - 04:23 AM.