9 replies to this topic

[Barathvaj]

I'm a student pursuing to become a researcher in the field of lifescience. .I'm student to all those who teaches me. I'm just trying to bloom so help me plz..

Can i know how to isolate a bacteria from soil and how to analyse its presence without the help of microscope. .

Give me some ideas and protocols !

thanks!

Edited by Barathvaj, 27 January 2012 - 11:55 PM.

[Kamran]

Take soil sample, make a suspension in sterile water and inoculate an aliquot  on any agar containing media. You would get macroscopic bacterial colonies after some days incubation at ambient termperature or 37°C.

[Phil Geis]

Barathjav, is your objective to isolating some/any bacterium from soil?  What do you mean by "analyse" its presence?

[Barathvaj]

How to know whether I've isolated particular type of bacteria or not?,and there is any test to find the characteristics of bacteria?

which is the best method in isolating the bacteria from the soil?

after inoculating into agar medium,how to separate of bacterial species

[Phil Geis]

Thns for the response.  Can you tell me the extent to which you are trained in microbiology?

[Barathvaj]

I've learned sterilization techniques ,
Inoculation -spread plate,streak plate,pour plate,
                   agar medium preparation,
                   serial dilutions,
                   wet and dry mounting,
                   and few things  .          

If you can guide me from basic,it will be very useful for me!!!!!! plz

[Phil Geis]

anything in bacterial identification?

[pito]

Barathvaj, on 30 January 2012 - 05:22 PM, said:

I've learned sterilization techniques ,
Inoculation -spread plate,streak plate,pour plate,
   agar medium preparation,
   serial dilutions,
   wet and dry mounting,
   and few things  .   

If you can guide me from basic,it will be very useful for me!!!!!! plz

Its very difficult to just start with identification of soil bacteria.
The best you can do is try to find a good textbook/paper on isolation/identification of soil bacteria. You can start looking for papers about this and try to find specific media for soil bacteria. + try to find some bacteria (species for ex) that are known as "soil bacteria".
Then you can look up what their "potential" is (their characteristics) by looking up those species in for example bergeys.
You can also buy "identification" kits based on these characteristics... like API-20 strips.

But keep in mind that identifying bacteria is often done by pcr , and not sure you can do this?

You can start by reading this:
http://www.jlindquis.../102bactid.html
http://www.ableweb.o...christopher.pdf
http://www.nslc.wust...nbacteria06.pdf
and chech the attachement I made. I posted it here before I think, but to be sure, I'll add it here again. Its a usefull flow chart to find out what bacteria you are working with.
But keep in mind: these days, if we need a 100% accurate (or 99%) we use PCR..


Keep in mind its not soo easy to just tell what kind of bacteria it is.
ANd often its more a proces of elimination rather then just saying: aha its this one...

Attached Files

•  flowcharts.pdf   826.91K   195 downloads

Edited by pito, 31 January 2012 - 02:33 AM.

[Phil Geis]

Excellent information Pito but I fear it exceeds the knowledge and capability of the poster and it's not clear what the indivudual is trying to accomplish.

[Barathvaj]

Oh ! very thanks for all! i'm just a beginer . .but whatever the info's U gave . It is useful ! .I'm a UG student but i like to become a researcher.so i'm trying to train myself by getting knowledge from all those who teaches me!

sorry for confusing you all before. .hereafter i'll make it clear

Edited by Barathvaj, 05 February 2012 - 06:32 PM.