high MW dimerswhat does it mean
Posted 27 January 2012 - 01:12 PM
I know maybe it could be another site the primers could've annealed to and amplified, but since it is a dimer and not a single high molecular weight band, I don't think it is another annealing site for sure. Could it be single stranded DNA? If it is, why does it happen that the PCR generates single stranded DNA ? Thanks
Posted 29 January 2012 - 07:51 AM
Dimer usually is a "single" band, didn't you mean smear?
I never trust anything that can't be doubted.
Posted 31 January 2012 - 01:37 AM