Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

high MW dimers

what does it mean

  • Please log in to reply
2 replies to this topic

#1 claritylight



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 104 posts

Posted 27 January 2012 - 01:12 PM

I ran a gel on my PCR product. I have a lot of my product at the right size, but also high molecular weight dimers at 2kb. What could this be possibly?

I know maybe it could be another site the primers could've annealed to and amplified, but since it is a dimer and not a single high molecular weight band, I don't think it is another annealing site for sure. Could it be single stranded DNA? If it is, why does it happen that the PCR generates single stranded DNA ? Thanks

#2 Trof


    Brain on a stick

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,360 posts

Posted 29 January 2012 - 07:51 AM

How do you know it's a dimer, did you sequence it? Does it appear in your negative control?
Dimer usually is a "single" band, didn't you mean smear?

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon

#3 akhshik


    Nice day for research

  • Active Members
  • PipPipPipPipPip
  • 30 posts

Posted 31 January 2012 - 01:37 AM

may be you need to optimize your PCR by increasing temperature or changing MgCl2 concentration.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.