I ran a gel on my PCR product. I have a lot of my product at the right size, but also high molecular weight dimers at 2kb. What could this be possibly?
I know maybe it could be another site the primers could've annealed to and amplified, but since it is a dimer and not a single high molecular weight band, I don't think it is another annealing site for sure. Could it be single stranded DNA? If it is, why does it happen that the PCR generates single stranded DNA ? Thanks
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#2
Trof
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Posted 29 January 2012 - 07:51 AM
How do you know it's a dimer, did you sequence it? Does it appear in your negative control?
Dimer usually is a "single" band, didn't you mean smear?
Dimer usually is a "single" band, didn't you mean smear?
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
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#3
akhshik
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Nice day for research
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Posted 31 January 2012 - 01:37 AM
may be you need to optimize your PCR by increasing temperature or changing MgCl2 concentration.
