2 replies to this topic

[sujatharaghu]

hi,

Could someone pl tell me  any good tutorial for  cloning a bacterial gene in E.coli, i would want to know right from the scratch like retrieving the bacterial sequence from NCBI,  i have lot of  doubts at every step, if someone could help me it would be nice, thnks....

[bob1]

Try Molecular Cloning: a laboratory manual, by Sambrook et al. - it is the "bible" for molecular biology.

[sujatharaghu]

thank you