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TEV purification unspecific band appearing

TEV

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#1 peppy

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Posted 25 January 2012 - 09:21 AM

Hey guys ,
i'm writing because every time i'm doing His-TEV purification, when I check the induction a non specific band comes up in the coomassie gel.Posted Image
His-TEv is 27kDa, this band is around 40-42 kDa.
I would exclude it's a dimer. Do you think it could be a glycosylated form?Posted Image

Thanks for your help





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