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yeast growth in SC-ura medium


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#1 deespike

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Posted 25 January 2012 - 08:35 AM

Hi all
I have suggest started working on yeast transformation and I am little bit confused with the result I obtained to that of theory.
My yeast mutant strain is not deficient in His and Trp, but uracil, and the plasmid I transformed into it has uracil so it shall complement for it in the nutrient medium deficient in uracil, His and TRP. I grew the mutant strain in SC-ura- medium( Yeast Nitrogen base, glucose only and no amino acids) without any plasmid so no growth was seen. Similarly, the mutant strain transformed with plasmid grew in the same medium.
Now, I am confused that since I have added no other AAs into the medium how is the transformed yeast growing in the medium. Can yeast grow in medium without amino acids and produce for its own?

#2 biocrazy

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Posted 25 January 2012 - 06:54 PM

Hi,

Do you use Uracil dropout mixture? If so it contains all the other amino acids except Uracil.




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