2 replies to this topic

[dan_]

Hi all,

I want to clone 2,2kb fragments into 15 kb vector by bacteria recombination, because I have tried several time by ''classic'' clonage (restriction enzyme and ligation) and it doesn't work.

Thanks,

[pDNA]

http://web.ncifcrf.g...ocol1_DY380.pdf

this should be a good starter!

Regards,
p

[dan_]

Thank you so much for your help, pDNA.

dan