Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Recombination protocol needed


  • Please log in to reply
2 replies to this topic

#1 dan_

dan_

    member

  • Active Members
  • Pip
  • 25 posts
0
Neutral

Posted 25 January 2012 - 07:43 AM

Hi all,

I want to clone 2,2kb fragments into 15 kb vector by bacteria recombination, because I have tried several time by ''classic'' clonage (restriction enzyme and ligation) and it doesn't work.

Thanks,

#2 pDNA

pDNA

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 492 posts
14
Good

Posted 26 January 2012 - 09:06 AM

http://web.ncifcrf.g...ocol1_DY380.pdf

this should be a good starter!

Regards,
p

#3 dan_

dan_

    member

  • Active Members
  • Pip
  • 25 posts
0
Neutral

Posted 27 January 2012 - 11:16 AM

Thank you so much for your help, pDNA.

dan




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.