fatty acid free BSA-cell cultureDear all
Posted 23 January 2012 - 04:43 PM
I need to treat my cells Huh7 with oliec acid to induce fatty liver model. I've read in articles about fatty acid free bovine serum albumin and how they use a combination of fatty acid and FAF-BSA. I am wondering what's the point of using FAF-BSA . Do u think using FAF-BSA will minimize the amount of oliec acid the cells will expose to (which somthing we must avoid it)? There must be a reason for using it....any help in details plzzzzz
It would be great if u could recommend some refrences...
Thanks in advance
Posted 24 January 2012 - 02:04 PM
Posted 25 January 2012 - 04:44 AM
Posted 25 January 2012 - 12:46 PM
Posted 31 January 2012 - 01:43 PM
Posted 19 February 2012 - 03:57 PM
Posted 10 July 2014 - 03:47 AM
I have also been treating cells with different types of FA (such as OA) and for this I dont use BSA. Like you, I see that people tend to use a combination of FAF-BSA however I could not find an explanation for why they do it like that. I have seen people saying that is a vehicle for lipids because it binds to the FA not allowing it to stick everywhere, well this makes sense if you adding it to living beings but if you using for cell cultures I dont see why it should matter and I will explain why:
1- If you dissolve your FA in for example Ethanol you can obtain an homogenous solution if you don't go above the solubility limit. This way you make sure you adding the amounts you want. You just need to be careful to the amount of Ethanol you adding to the cultures (ex. for COS cells you should not go above 1% (v/v)). Of course high FA concentrations are toxic to the cells but you would need to go very high since cells have mechanisms to protect against lipotoxicity (ex: storing lipids in LDs). Thid also depends in the type of cells you using.
2- If you use BSA and it binds to FA how much FA is going to diffuse to the cells? Also, if you have a BSA-FA complex this is probably less permeable to the cell membrane than the FA. In addition, cell membranes have FA transports.
In terms of references I cant help you there.