6 replies to this topic

[shs]

Dear All,

I need to treat my cells Huh7 with oliec acid to induce fatty liver model. I've read in articles about fatty acid free bovine serum albumin and how they use a combination of fatty acid and FAF-BSA. I am wondering what's the point of using FAF-BSA . Do u think  using FAF-BSA will minimize the amount of oliec acid the cells will expose to (which somthing we must avoid it)? There must be a reason for using it....any help in details plzzzzz
It would be great if u could recommend some refrences...

Thanks in advance

[shs]

No help

[bob1]

It would be so that you can control the levels of oleic acid - that way you can determine if the effect is dependent on the amount of oleic acid present and/or if the effect is due to oleic acid alone.

[shs]

Thank u for ur replay but can u explain more

[bob1]

Presumably you are going to add oleic acid to your cells to induce the fatty liver model, as you mentioned in your first post?  Oleic acid is a fatty acid - so if you use ordinary BSA, how do you know how much oleic acid it has?  If you use FAF-BSA, you can add a defined amount of oleic acid, and thereby determine that the effect you are seeing is due only to the oleic acid you added, not some already present in the BSA or from some other component in the BSA.

[klinmed]

BSA is used as a carrier of long- chain fatty acids. High concentration of free (unbound) FA are generally quite toxic for cells in culture.

[shs]

Many Thanxxxx