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PCR buffer without Tris

Started by Priyanka Rathi, Jan 23 2012 11:45 AM

PCR BUFFER TRIS

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#1 Priyanka Rathi

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Posted 23 January 2012 - 11:45 AM

Hi Guys

I am trying to do a PCR.I need to conserve the pH changes so I want to make a buffer without Tris ,so that H-ion is not buffered...I have had papers regarding this..but as they have mentioned its not working.I have tried many times ,its failure always.I make buffer with ph 8.

Any sugggestions?

Thanks
Priyanka

#2 akhshik

Nice day for research

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Posted 31 January 2012 - 01:42 AM

what are you using instead of Tris? most of buffers ruins your PCR

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