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PCR buffer without Tris

PCR BUFFER TRIS

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#1 Priyanka Rathi

Priyanka Rathi

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Posted 23 January 2012 - 11:45 AM

Hi Guys

I am trying to do a PCR.I need to conserve the pH changes so I want to make a buffer without Tris ,so that H-ion is not buffered...I have had papers regarding this..but as they have mentioned its not working.I have tried many times ,its failure always.I make buffer with ph 8.

Any sugggestions?

Thanks
Priyanka

#2 akhshik

akhshik

    Nice day for research

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Posted 31 January 2012 - 01:42 AM

what are you using instead of Tris? most of buffers ruins your PCR





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