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Three clones of a cell line?

cell biology molecular biology clones

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3 replies to this topic

#1 Yasar

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Posted 22 January 2012 - 02:08 PM

Hi,
I am looking at BCR/ABL expression of two philadelphia positive cell lines. According to plan, I have to use three clones of each cell line and do a PCR in triplicates. How long do I have to grow a cell line in three different flasks to call them different clones of a cell line? Is there any guideline or definition
Thanks
Yasar

#2 Kamran

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Posted 23 January 2012 - 04:07 AM

Three clones of a single cell means, you pick up single cell from each cell line, grow those single cells to a desired cell density and perform your experiment.

#3 Yasar

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Posted 25 January 2012 - 03:09 PM

Hi Kamran,
Thank you very much for your reply. how would I pick one cell from an aliquot of cells and how would I grow them? Is there any protocol for that? I thawed three frozen vials of a cell lines and grew them separately. Would you consider them as three clones?
Thanks.

#4 bob1

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Posted 26 January 2012 - 12:00 PM

No, three vials are not considered clones. Have a look for protocols relating to limited dilution and/or colony selection.





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