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cloning of fruit specific promoter

promoter function analysis cloning expression Transformation

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#1 doctormuhandas



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Posted 21 January 2012 - 11:22 AM

Dear All,

I am new at molecular biology. I have isolated a fruit specific promoter with the help of PCR. It showed exact expected size on gel 2120 bp. I want to use this to make a construct in which my gene of interest is controlled by this fruit specific promotor so that i can get expression specifically in the fruit. My problem is that how can i identifiy that this promoter is funtional before transformation of plants. Is there any way? Your help will be highly appreciated. Thanks.


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