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cloning of fruit specific promoter

promoter function analysis cloning expression Transformation

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#1 doctormuhandas

doctormuhandas

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Posted 21 January 2012 - 11:22 AM

Dear All,

I am new at molecular biology. I have isolated a fruit specific promoter with the help of PCR. It showed exact expected size on gel 2120 bp. I want to use this to make a construct in which my gene of interest is controlled by this fruit specific promotor so that i can get expression specifically in the fruit. My problem is that how can i identifiy that this promoter is funtional before transformation of plants. Is there any way? Your help will be highly appreciated. Thanks.

Doctormuhindas





Also tagged with one or more of these keywords: promoter, function analysis, cloning, expression, Transformation

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