Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

cloning of fruit specific promoter

promoter function analysis cloning expression Transformation

  • Please log in to reply
No replies to this topic

#1 doctormuhandas

doctormuhandas

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 21 January 2012 - 11:22 AM

Dear All,

I am new at molecular biology. I have isolated a fruit specific promoter with the help of PCR. It showed exact expected size on gel 2120 bp. I want to use this to make a construct in which my gene of interest is controlled by this fruit specific promotor so that i can get expression specifically in the fruit. My problem is that how can i identifiy that this promoter is funtional before transformation of plants. Is there any way? Your help will be highly appreciated. Thanks.

Doctormuhindas





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.