I am new to ICC and have a basic question. How do you determine incubation time and temperature for antibodies? It seems to vary a lot, esp. for primary, ranging from 1 hr at 37C to 1-2 days at 4C...What works better? Or does it depend on which proteins you are staining for?
Edited by sc_queen, 21 January 2012 - 11:01 AM.