Immunohistochemistry: What am I looking at?immunohistochemistr
Posted 20 January 2012 - 06:33 PM
But I really don't quite understand what I'm looking at. I understand how IHC works- the binding of the primary to the desired protein and then the binding of the secondary tagged with a fluorphore to the primary, etc. But I suppose I don't recognize the tissue behind the staining? Is IHC mainly used to determine the presence or absence of a protein of interest? I can do that, although I wouldn't be able to tell where exactly the protein is. Can you glean any more information from IHC?
I'm using sections from a mouse. I've looked up some pictures of what tissue sections from them are supposed to look like, but, after staining, the tissue looks nothing like them. How do people read these things? Any advice or helpful websites, books, etc would be greatly appreciated.
Posted 20 January 2012 - 07:56 PM
To be able to view IHC slides, I assume you have done counterstaining which makes cellular structuer clear to see, you need to have some knownledge about the organ or tissue you are working with. This is usually taught in histology class. There are many online resources providing images of mouse or human HE stained tissue sections, such as this one The Visible Mouse Project at http://tvmouse.compmed.ucdavis.edu/
Posted 21 January 2012 - 07:03 PM