can any one tell me about cholestrol determiantion in blood
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#2
Bluefunk
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Posted 16 February 2012 - 07:54 PM
When we measure cholesterol, we extract all the lipids in the cell using a bligh and dyer differential extraction (google it, its simple). In our lab, we use an Iatroscan TLC-FID (thin layer chromatography flame ionization detector), which separates the lipids and when compared to standards, quantifies them by detecting the ions formed when it is burnt).
#3
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Posted 17 February 2012 - 07:08 AM
in vitro assays based on cholesterol oxidase reaction
