Posted 28 January 2012 - 02:35 AM
Having one StepOne and later upgraded to stepone plus in the department, I will not recommend you to get this machine.
1) As far as I understand, You need to purchase ABI plates, strips, seal etc in order to use StepOne system. You unable to use other brand's (Axygen etc..) for StepOne. They are using 0.1ml strips which other manufacturer's do not fit, last time I try (I have to admit that is very long time ago). So, using StepOne as normal PCR machine will increase your research cost.
2) Agree that StepOne you can program your run on the qPCR machine itself without the need of additional computer, and thats the only thing that you can do. After your qPCR run, and if you do not save your run data immediately, you will lost all your running data if someone else start their run and did not save yours, as there is no internal memory for you to do so. (I would say this is a very stupid design by ABI, your need to end up plugging your thumbdrive on the machine when you do your run). You can install the StepOne client on your own lab PC for your qPCR configuration and data analysis. FYI, HRM analysis requires you to purchase another software (dongle) in order to run the HRM, else it will be handicapped.
Think twice before you get this StepOne machine, I totally not recommend it. You can try stratagene MX3000, I "heard" (never use before) is a very good one.
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..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
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