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Immunocytochemistry with peroxidase

Started by quentind, Jan 20 2012 06:48 AM

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#1 quentind

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Posted 20 January 2012 - 06:48 AM

Hi everyone,
I am a newby in immunostaining, and my lab has no experience about it.
I would like to test actin immunostaining on my HEK cells, but I don't have any fluorescent secondary antibodies, but I have peroxydase antibodies that I use with Amersham ECL for my WB.
Have you ever used this in immunostaining?
Thanks a lot

#2 bob1

Thelymitra pulchella

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Posted 20 January 2012 - 03:12 PM

The DAB based systems are peroxidase reactions. You will find ICC on 293's difficult as they don't adhere well to slides/coverslips and wash off during processing.

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