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miRNA extraction from cell-conditioned media?

Started by miR-girl, Jan 20 2012 05:11 AM

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#1 miR-girl

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Posted 20 January 2012 - 05:11 AM

Has anyone had any success extraction miRNA from cell-conditioned media?

Theres very little reported evidence of it out there. Some have used miRNeasy and miRVana, but yields are extremely low.

#2 akhshik

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Posted 31 January 2012 - 02:03 AM

I heard that in a group the have done it as 2 part method: 1st extract Mitochondria and then extract RNA from them.
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