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HELP : FITC conjugated BSA

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#1 kaul thomas

kaul thomas


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Posted 19 January 2012 - 09:35 AM

I am using fluorescent plate reader to calculate the fluorescent intensity of FITC BSA . I want to know how can we quench the fluorescent intensity of FITC BSA, either physically or chemically without changing the original color of FITC BSA solution (orange).

I read that FITC (without BSA conjugation) decomposes in water (ref : sigma ), .?

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