big differnece in my primers Tm
Posted 19 January 2012 - 02:25 AM
I have to do cloning and I have cDNA but the problem is with primers, I designed the primers and eventually one of them has Tm 66 the other is 80, I did gradient PCR with control DNA and I have very small barely seen band at 76, can i proceed my experiment though or should I redesign my primers?
any advice will be appreciateed.
Thank you so much in advance.
"Be the change that you wish to see in the world"
Posted 19 January 2012 - 09:55 AM
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon
Posted 19 January 2012 - 10:48 AM
Edited by hobglobin, 19 January 2012 - 10:49 AM.
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.