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Housekeeping gene


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#1 navadann

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Posted 24 April 2003 - 07:13 AM

I am performing a southern blot on gDNA but have failed to get a signal with my probe. i am beginning to think it may be the probe (900bp). i was wondering what housekeeping gene probe i can use just to make sure the problem technically the southern is working. Will gapdh work? thank you

#2 hennry

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Posted 26 May 2003 - 09:37 AM

Hi,
if you just want to know if your blot technique is working i'd recommend dot blots with your labelled and unlabelled probe and a probe you know for sure that it's working (i used my commercially available DIG-labelled lambda-marker). Worked well for me during colony filter lifts to find out that my dNTP-labelling mix did not work.
Regards,
Henry




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