Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Annexin binding buffer (urgent advice)

concentration preparation

  • Please log in to reply
2 replies to this topic

#1 madelingirly

madelingirly

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 111 posts
4
Neutral

Posted 18 January 2012 - 09:10 PM

Dear Guys,
I am new in flow cytometry and now one in my lab has any experience in it too,
I want to prepare annexin binding buffer, I found its concentration, but what I cant find are these concentration per not.
Could any one verify this meaning for me please.
I have attached the protocol of my Annexin-V fluos the reagent I have and it is written that I should have a binding buffer :
10mMole HEPEs.
140mMole NaCl, and 5mMole CaCl2.
my question these quantities per liter or what????
Sorry for silly question, but it made me confused.
Thank you in advance
Shimaa

#2 keefec

keefec

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 20 February 2012 - 12:04 AM

These quantities should be in litre.

#3 almost a doctor

almost a doctor

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 244 posts
15
Good

Posted 20 February 2012 - 02:03 AM

These quantities should be in litre.


That's not strictly true.

I assume when you say mMole you actually mean mMolar (or mM). In this case the final volume is irrelevant, whether is 1ml or 1litre of 10mM Hepes, it will still be 10mM. You do of course need to know the volume to prepare the reagents, but what will change is the amount of reagent (in grams) you need, the concentration should be the same. So basically, choose your required volume and calculate from there.

Now, if you are talking about mmoles is a different story, but I doubt is the case as buffers are always referred to in Molar concentration.

Edited by almost a doctor, 20 February 2012 - 02:03 AM.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.