Is a round-bottomed plate fine for measuring BCA using a plate reader?
How does the light (emission or absoption?) work in these machines?
Also, is a non-transparent one ok? or does it have to allow light to pass through the plate?
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Types of 96-well plates used for BCA protein quantification
Started by science noob, Jan 18 2012 07:36 PM
1 reply to this topic
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Hmmm, I think it's working
Posted 19 January 2012 - 01:02 PM
You need a transparent flat bottomed plate to read the concentration effectively by absorbance (BCA is an absorbance assay - you are measuring at only one wavelength, no excitation and emission). Round bottomed plates scattter the light too much to be effective for the reading. Non-transparent plates don't work with absorbance for obvious reasons!
