Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Need replacement spin columns for Qiagen's Blood and Tissue DNeasy Kit


  • Please log in to reply
3 replies to this topic

#1 grim

grim

    member

  • Members
  • Pip
  • 3 posts
1
Neutral

Posted 18 January 2012 - 11:25 AM

We still have all the buffers necessary for Qiagen's DNeasy Blood and Tissue kit, and they're all still fresh as well. We are just out of the DNeasy Mini Spin columns.

To order more spin columns from Qiagen requires you to purchase the rest of the kit as well, which is unnecessary and ridiculously expensive. However, I found these: http://www.epochlife...n/minispin.aspx

Has anyone had any experience with these columns, and do they work as well? I'm going to request a free sample but if anyone's got a review of this product I'd love to hear it.

Thanks!

#2 S.Linares

S.Linares

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 16 January 2014 - 03:00 AM

I am actually having the same problem.

Did you ever try those columns? If so, did they work?, and what is the nature of your sample?



#3 jerryshelly1

jerryshelly1

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 328 posts
33
Excellent

Posted 17 January 2014 - 07:59 AM

Email the company, but yes they should work. Silica columns are not really proprietary and every company uses the same one. The columns may differ in the grade of plastic and the design, but the principle will be the same. 



#4 anderson

anderson

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 02 December 2014 - 02:24 PM

I posted in another thread that we have been using Epoch Life Science's spin column for the past seven years. Mainly for plasmid, PCR cleanup and gel extraction. We make our own buffer according to their recipe. Never had any problem. But they don't provide buffer recipe for blood tissue DNA extraction. We were able to figure out our own recipe for some other appliacations buy following google scholar, aparently many other labs are using Epoch's mini spin columns:

http://scholar.googl...=en&as_sdt=0,44
http://scholar.googl...=en&as_sdt=0,44

If you have old buffers from Qiagen's DNeasy Blood and Tissue kit, Epoch's column will work beautifully.

They provide free samples, just email them. We received 20 some samples when we first tried it.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.