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Purification of Sequence-Specific DNA-Binding Proteins by Affinity Chromatograph

Purification

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#1 memari

memari

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Posted 17 January 2012 - 02:32 PM

I want to make an oligonucleotide for Purification of Sequence-Specific DNA-Binding Proteins by Affinity
Chromatography. I just want to know is there any tips and tricks for DESIGNING the oligonucleotide like designing Primers? for example
how long should it be?
How many times the Sequence-Specific DNA should be repeated?
How long the flanking DNA should be ? and its content? CG rich or AT rich?
Do Nucleases interfere and digest my immobilized oligo-nucleotides?
Which way is better for coupling the oligo-nucleotides to resin and which resin is better? Cyanogen Bromide is better or binding of biotin to streptavidin?

how to choose the best Sequence-Specific DNA and which websites can help me?

I would be appreciate...
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Babak Memari





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