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How to determine whether Adenosine A2B receptor is inhibited by WB

Western Blot Adenosine

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#1 Benchworker



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Posted 17 January 2012 - 09:12 AM

Hi, All

I need some help about how to determine if Adenosine A2B receptor is inhibited by its specific blocker MRS 1754 in H9c2 cells or adult-rat cardiomyocytes by using Western Blot? I cannot find specific downstream proteins to detect which are unique to A1/A3 receptors' which also locate in these types of cells.


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