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Determine whether Adenosine A2B receptor is blocked by its inhibitor

Adenosine Inhibitor

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#1 Benchworker

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Posted 17 January 2012 - 09:07 AM

Hi, All

I need some help about how to determine if Adenosine A2B receptor is blocked by its specific inhibitor MRS 1754 in H9c2 cells or adult- rat cadiomyocytes. Any methods??


Thanks!

#2 Inmost sun

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Posted 02 March 2012 - 07:54 AM

classically done with radioactive-labelled and non-labelled ligand for ligand-binding curves; non-radioactive method f.i. is surface plasmon resonance





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