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Elisa


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#1 scompton

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Posted 22 April 2003 - 08:41 AM

I'm doing a sandwich Elisa. I incubate the secondary Ab in the same solution that I dilute my samples with :1% BSA, 1% Casein, PBS .05%Tween-20. The assay Im doing is really sensitive and for the most doesnt seem to be working. Also, if Im using OPD how much stopping solution should I use?? Equal amount? Now I'm using 100uL OPD and 50uL stopping solution.
Thanks
Sharon

#2 wkchand

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Posted 26 May 2003 - 07:11 PM

What do you mean of 'too sensitive'? Actually, ELISA is a rapid and sensitive method. I guess you mean the backgroud is over. Have you tried to use different combinations of Primary Ab and Secondary Ab at various concentration yet? To stop the reaction, I also used 50ul stopping solution.




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