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Freeze and Thaw for cell lysis

Freeze thaw cell lysis protein

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#1 Vinoth Shanmukam

Vinoth Shanmukam

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Posted 17 January 2012 - 08:37 AM

Could you please anybody give stepwise procedure for doing Freezing and thawing for Mammalian cell lysis??

Do I need to take only cells or cells with pbs or with lysis buffer???

After freezing and thawing.. I should take the lysate???

Thank you,
Vinu

Edited by Vinoth Shanmukam, 17 January 2012 - 09:13 AM.






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