MTT problems (Seeking advice)cytotoxic agent
Posted 17 January 2012 - 12:06 AM
At first I am very grateful for every one who will read this topic
and deeply indebted for those who will share their experience, thoughts with me .
Today was my first MTT assay on Tongue squamous cell carcinoma
I used a cytotoxic agent which is ZnCl2 to establish standard curve.
I used 10 microliter of the following concentration :
I followed the protocol of MTT assay as widely present online
what was strange that higher concentration showed a stronger purple color ( higher viability).
While low concentration showed a lower absorbance.
I wonder if any one could share his experience regarding using standard cytotoxic agents ( Not drugs ).
Posted 02 April 2012 - 08:44 PM
I will write the answer perhaps someone else face the same problem.
The problem was I didn't filter my MTT solution (I weighted the powder and dissolve it in PBS), so it was not sterile which lead to inaccurate results.
So sterilization of your MTT solution is very important to consider during your experiment as contamination lead to inaccurate results
Posted 28 July 2012 - 06:17 AM