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Is Tris-HEPES a good substitute for Tris-HCl?


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#1 Laforet

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Posted 16 January 2012 - 05:47 PM

We routinely use Tris-HCl at pH8 for our buffers but we know that it's pH changes a lot with temperature. Recently I have noticed that a solution of 50mM Tris base and 50mM HEPES acid has a pH of 8.0 anywhere between 4-25C. Is it possible to replace Tris-HCl with Tris-HEPES if the chloride concentration is not an issue?

Besides, can someone tell me the correct way to calculate the buffering range/capacity of a mixed buffer like Tris-HEPES?

#2 bob1

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Posted 17 January 2012 - 11:31 AM

If you just want a buffered solution of a particular pH, then tris-hepes could work, as could phosphate buffered....




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