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Preparation of tibia or femur for microCT


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#1 Alex_3

Alex_3

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Posted 16 January 2012 - 06:45 AM

Hi everybody,

Actually I'm looking for convenient protocols for preparation of tibia and femur of mice. The bone will be analyzed by micro CT for determination of the trabecular bone volume and by pq-CT for bone mineral density.
I suggest that these protocols are quite simple, however I'm uncertain whether I should use protocols utilizing PFA for fixation, just Ethanol or even KOH to get rid of connective and muscle tissues. In addition, I’m not sure about storing the bone before CT at 4°C or -20°C ?

Thanks
Alex




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