I have a trouble in PCR with phophorylated primers.
I used FAM-Forward Primer and Phophorylated reverse primer. (Template < 100bp).
As a Polymerase, Hotstar Taq polymerase is used for our PCR (30 cycles).
My issue is that after PCR (30 cycles),
just FAM Forward Primer and Phophorylated reverse Primer showed the unexpected band
(pretty similar location to the Template DNA+Complementary DNA band) without template and complementary DNA.
Do you know why these bands are existed?
Could you please help me to understand why?
Edited by Ju Hun, 14 January 2012 - 04:42 PM.