a band that suppose to be 90KD shows up at 70KD!!
Posted 13 January 2012 - 12:50 PM
I have been coming a cross some unexplained blots. My target protein is suppose to be coming around 90 KD, but every time I run a WB it shows up at 70KD. I am following the general protocol for WB with very small changes. I called the company and they recommend to remove Tween from all of the buffers I am using, but this is didnt fix the problem and I am still seeing the bands at 70 KD.. any suggestion of why this is happening and how to fix it !!
Posted 19 January 2012 - 08:41 AM
if your result is consistent and you are certain that you are detecting the correct protein then i wouldn't worry too much.
Edited by mdfenko, 19 January 2012 - 08:42 AM.
genius does what it must
i do what i get paid to do