Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

native protein expression in bacteria (e.coli)

  • Please log in to reply
No replies to this topic

#1 passenger33



  • Members
  • Pip
  • 1 posts

Posted 13 January 2012 - 11:47 AM


Currently, i have a gene of interest that is in a pET22b vector that i use for protein purification with a BL21 e.coli system.

However, now i want to introduce that gene of interest into another strain of E.coli that is typically used for antibacterial activity. I want the gene of interest to be natively expressed by the bacteria.
But since the strain of e.coli i want to use does not rely on the T7 expression system (and consequently IPTG induction), i need to find a different vector that will promote native bacterial expression and give a basal amount that i can use for an antibacterial assay.

Does something like that exist? Bacteria regularly exchange plasmids through horizontal transfer, so there much be a plasmid that i can exploit to make bacteria express a protein that i want.

Many thanks!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.