The western blot was done for CHO, COS-7 and NIH3T3 cell lysates which were also treated with DFP ( a protease inhibitor) using the insulin receptor beta antibody (C-19, purchased from Santa Cruz). I obtained the required band at 95kDa confirming the presence of the insulin receptor beta subunit in these lysates. But, why do I see an extra band at around 60 kDa also? What could be the reason behind that?
Edited by sansub, 12 January 2012 - 04:24 PM.