Hi,
I have a plasmid of about 7.5 kbp that needs to be sequenced. Due to shortage of time I don't really have time to design a primer, and so my supervisor and I wondered if it was possible to sequence the whole plasmid without a primer.
Are there companies that can do that?
Is that even possible?
Hope anyone is able to help me with this!
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1 reply to this topic
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mdfenko
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an elder
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Excellent
Excellent
Posted 12 January 2012 - 08:37 AM
you may not need to design a primer. commercial plasmids have standard priming sites designed in. just obtain the standard primer closest to your insert (from a neighbor?) and sequence away.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
