2 replies to this topic

[estudiantebiochem]

Hi, I am a Biochem student.  I am extracting DNA from plants that have been exposed to contaminants, then using restriction enzymes to see if there is any change.  I was wondering if there was a difference between dried sample results and a fresh sample? Thanks

(Edited by estudiantebiochem at 2:10 am on July 12, 2001)

[AronD]

estudiantbiochem,

As long as you handle the plant tissue properly, there should be no difference between your fresh and dried samples.  This means that you should either freeze your tissue at -70C to -80C or lyophilize your tissue immediately after you harvest it.  This will minimize DNase activity in the plant tissue before you extract the DNA.

AronD

[estudiantebiochem]

Thanks