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Plant DNA extraction


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#1 estudiantebiochem

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Posted 12 July 2002 - 07:07 AM

Hi, I am a Biochem student.  I am extracting DNA from plants that have been exposed to contaminants, then using restriction enzymes to see if there is any change.  I was wondering if there was a difference between dried sample results and a fresh sample? Thanks

(Edited by estudiantebiochem at 2:10 am on July 12, 2001)


#2 AronD

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Posted 12 July 2002 - 11:34 AM

estudiantbiochem,

As long as you handle the plant tissue properly, there should be no difference between your fresh and dried samples.  This means that you should either freeze your tissue at -70C to -80C or lyophilize your tissue immediately after you harvest it.  This will minimize DNase activity in the plant tissue before you extract the DNA.

AronD


#3 estudiantebiochem

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Posted 12 July 2002 - 05:48 PM

Thanks




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