Hi all,
do any of you know what techniques can be used to determine protein quantity in cell lysates that contain 2% triton x. I have lysed my cells in lysis buffer containing 2% triton, because I am looking at a membrane bound protein (LC3) by WB.
Bradford etc. reagents are reacting very! good with Triton X, and the reagent gets saturated very fast by Triton.
Any suggestions?
0
1 reply to this topic
#2
mdfenko
-
- Active Members
-
- 2,241 posts
an elder
77
Excellent
Excellent
Posted 12 January 2012 - 08:22 AM
the bca protein assay is compatible with up to 5% triton x-100 (1% other tritons)
Edited by mdfenko, 12 January 2012 - 08:24 AM.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
