Protein measurements in 2% Triton X samples
Posted 11 January 2012 - 08:22 AM
do any of you know what techniques can be used to determine protein quantity in cell lysates that contain 2% triton x. I have lysed my cells in lysis buffer containing 2% triton, because I am looking at a membrane bound protein (LC3) by WB.
Bradford etc. reagents are reacting very! good with Triton X, and the reagent gets saturated very fast by Triton.
Posted 12 January 2012 - 08:22 AM
Edited by mdfenko, 12 January 2012 - 08:24 AM.
genius does what it must
i do what i get paid to do