Hi all,
do any of you know what techniques can be used to determine protein quantity in cell lysates that contain 2% triton x. I have lysed my cells in lysis buffer containing 2% triton, because I am looking at a membrane bound protein (LC3) by WB.
Bradford etc. reagents are reacting very! good with Triton X, and the reagent gets saturated very fast by Triton.
Any suggestions?
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mdfenko
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Excellent
Excellent
Posted 12 January 2012 - 08:22 AM
the bca protein assay is compatible with up to 5% triton x-100 (1% other tritons)
Edited by mdfenko, 12 January 2012 - 08:24 AM.
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