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Proteominer on VERY Low Protein Levels

Proteomics Proteominer Low abundance Vitreous Purification

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#1 oflynnde

oflynnde

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Posted 11 January 2012 - 07:52 AM

Hi all,

I use Proteominer Enrichment kits on serum samples all the time and recently I've started working on vitreous fluid. Unfortunately, both the sample volume and concentration of the fluids are low so I can't process them using the ordinary kits.

I would, however, like to try and use a small amount of beads, relative to the sample concentration and volume, from the column to process the samples. Initially I plan on optimising this for 2 samples. The total concentration of one is 2.23mg and I have 250uL. The other sample has about 0.4mg and I only have 120uL of this.

I understand that this is difficult task but it would be great if I could at least try this and see the results. Does anyone have any tips or ideas?

Thanks.

Edited by oflynnde, 11 January 2012 - 07:54 AM.






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