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RE site on complimentary strand of plasmid?


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#1 sevendoctor

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Posted 11 January 2012 - 06:05 AM

Hi everyone,
Sorry if this has been posted before (or is a stupid question) but cant seem to find any information on this!

I want to digest a plasmid with a specific RE...but the RE site is on the comp strand of my plasmid...should this be a problem and will I have to take anything else into consideration?

Thanks for your help! Posted Image

#2 bob1

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Posted 15 January 2012 - 11:24 AM

Most RE sites are totally complementary - i.e. if it is in one strand, then it should be in the other too, just in the reverse direction!




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