Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

RE site on complimentary strand of plasmid?

  • Please log in to reply
1 reply to this topic

#1 sevendoctor



  • Members
  • Pip
  • 3 posts

Posted 11 January 2012 - 06:05 AM

Hi everyone,
Sorry if this has been posted before (or is a stupid question) but cant seem to find any information on this!

I want to digest a plasmid with a specific RE...but the RE site is on the comp strand of my plasmid...should this be a problem and will I have to take anything else into consideration?

Thanks for your help! Posted Image

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,506 posts

Posted 15 January 2012 - 11:24 AM

Most RE sites are totally complementary - i.e. if it is in one strand, then it should be in the other too, just in the reverse direction!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.