Im sonfused about :
Which step that significantly reduces the yield of your product protein?
What are the important factors in performing Bradford assay correctly?
Is BSA good to use for my standard curve ?
Thanks for ur help !
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4 replies to this topic
#2
kottila
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Posted 11 January 2012 - 04:48 AM
1.?
2. stay in the "linear" part of the assay, dilute your samples if necessary.
3. That's the most used protein for creating a standard curve.
2. stay in the "linear" part of the assay, dilute your samples if necessary.
3. That's the most used protein for creating a standard curve.
#3
mdfenko
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Posted 11 January 2012 - 07:57 AM
3. you may find that bovine gamma globulin (bgg) is a better standard for comparison with most proteins when performing the bradford assay (bsa gives ~2x the response of bgg and many other proteins).
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#4
YanYan
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Posted 13 January 2012 - 05:23 AM
In the first question, I think incubation step is too long so it leads to death phase , is it right dear ?
#5
Inmost sun
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Posted 28 February 2012 - 12:31 PM
1. which system?
3. add. to mdfenko: lysozym or artificial protein mix
3. add. to mdfenko: lysozym or artificial protein mix
