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co-transfection protocol

co-transfection

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#1 Raquelina

Raquelina

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Posted 11 January 2012 - 03:14 AM

Hi everybody!

I need a little help with an experiment. I'm trying to co-transfect my construction and another reporter plasmid at the same time. For transfecting the single construction, I use 10 ug DNA, my question is: should I put the same amount of reporter plasmid, 10ug? Since with the reporter I want to reflect the transfection efficiency I think the conditions should be the same, but I don't find any protocol.

Thanks in advance!




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